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rabbit anti-cb2 antibody  (Cayman Chemical)


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    Cayman Chemical rabbit anti-cb2 antibody
    Rabbit Anti Cb2 Antibody, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-cb2/rabbit+anti+cb1/pm38673761-197-19-23
    Average 90 stars, based on 1 article reviews
    rabbit anti-cb2 antibody - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Synthetic cannabinoids reduce the inflammatory activity of microglia and subsequently improve neuronal survival in vitro.
    Article Snippet: Microglia are resident immune cells of the brain that survey the microenvironment, provide trophic support to neurons, and clear debris to maintain homeostasis and healthy brain function.. Microglia are also drivers of neuroinflammation in several neurodegenerative diseases.. Microglia produce endocannabinoids and express both cannabinoid receptor subtypes suggesting that this system is a target to suppress neuroinflammation.

    Article Title: Opposing control of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis: in vitro and in vivo evidence.
    Article Snippet: Beside cytotoxic mechanisms impacting on neurons, amyloid (A )-induced astroglial activation is operative in Alzheimer’s disease brain, suggesting that persistent inflammatory response may have a role in the illness and that positive results may be achieved by curbing the astroglial reaction.. Because the role of the endocannabinoid system could represent a promising field of research, the present study conducted in vitro and in vivo experiments to assess this system.. C6 rat astroglioma cells were challenged with 1 g/ml A 1-42 in the presence or absence of selective agonists and antagonists of cannabinoid (CB)1 and CB2 receptors.

    Article Title: Cannabidiol and Oxygen-Ozone Combination Induce Cytotoxicity in Human Pancreatic Ductal Adenocarcinoma Cell Lines
    Article Snippet: Lysates were separated on a SDS polyacrylamide gel, transferred onto Hybond-C extra membranes (GE Healthcare, Chicago, IL, USA), blocked with 5% low-fat dry milk in phosphate-buffered saline 0.1% Tween 20 overnight at 4 °C, immunoblotted with mouse anti-CB1 (1:500, Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-CB2 (1:200, Cayman Chemical, Ellsworth, MI, USA), mouse anti-TRPV1 (1:200, Santa Cruz Biotechnology), mouse anti-TRPV2 (1:200, Santa Cruz Biotechnology), rabbit anti-TRPV3 (0.5 μg/mL, Boster Biological Technology, Pleasanton, CA, USA), rabbit anti-TRPV4 (1:500, Assay Biotechnology Company, Fremont, CA, USA), goat anti-TRPA1 (1:300, Santa Cruz Biotechnology), rabbit anti-TRPM8 (0.5 μg/mL, Boster Biological Technology), mouse anti-glyceraldehydes-3-phosphate dehydrogenase (GAPDH, 1:3000, OriGene, Rockville, MD, USA), and rabbit anti-caspase-3 (1:1000, Cell Signaling, Danvers, MA, USA) Abs overnight or 1 h according to manufacturer’s protocol and then incubated with their respective HRP-cojugated anti-rabbit, anti-mouse (1:2000, Cell Signaling, Danvers, MA, USA) or anti-goat (1:1000, Santa Cruz Biotechnology) Abs for 1 h. Peroxidase activity was visualized with the LiteAblot ® PLUS or TURBO (EuroClone, Milan, Italy) kit and densitometric analysis was carried out by a Chemidoc using the Quantity One software version 4.6 (Bio-Rad, Milan, Italy).

    Article Title: The fatty acid amide hydrolase inhibitor oleoyl ethyl amide counteracts bladder overactivity in female rats.
    Article Snippet: Giorgio Gandaglia,* Frank Strittmatter, Giovanni La Croce, Fabio Benigni, Arianna Bettiga, Fabio Castiglione, Marco Moschini, Francesco Mistretta, Christian Gratzke, Francesco Montorsi, Christian Stief, and Petter Hedlund Urological Research Institute, San Raffaele Scientific Institute, Milan, Italy Department of Clinical and Experimental Pharmacology, Lund University, Lund, Sweden Department of Urology, Munich University, Munich, Germany Department of Clinical Pharmacology, Link€ oping University, Link€ oping, Sweden

    Article Title: The endogenous cannabinoid system in the gut of patients with inflammatory bowel disease.
    Article Snippet: Membranes were blocked with 10 % nonfat dried milk and 5 % bovine serum albumin for 2 h, and then incubated overnight with rabbit anti-CB1 (diluted 1:500, Cayman Chemicals, Ann Arbor, MI), rabbit anti-CB2 (1:300, Cayman Chemicals), and mouse anti- -actin (1:1,000), mouse anti-STAT4 (1:500), mouse anti-phospho-STAT4 (pSTAT4, 1:500), and mouse anti-T-bet (1:500), all from Santa Cruz Biotechnology (Santa Cruz, CA).

    Activity Assay:

    Article Title: Synthetic cannabinoids reduce the inflammatory activity of microglia and subsequently improve neuronal survival in vitro.
    Article Snippet: Microglia are resident immune cells of the brain that survey the microenvironment, provide trophic support to neurons, and clear debris to maintain homeostasis and healthy brain function.. Microglia are also drivers of neuroinflammation in several neurodegenerative diseases.. Microglia produce endocannabinoids and express both cannabinoid receptor subtypes suggesting that this system is a target to suppress neuroinflammation.

    Article Title: Opposing control of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis: in vitro and in vivo evidence.
    Article Snippet: Beside cytotoxic mechanisms impacting on neurons, amyloid (A )-induced astroglial activation is operative in Alzheimer’s disease brain, suggesting that persistent inflammatory response may have a role in the illness and that positive results may be achieved by curbing the astroglial reaction.. Because the role of the endocannabinoid system could represent a promising field of research, the present study conducted in vitro and in vivo experiments to assess this system.. C6 rat astroglioma cells were challenged with 1 g/ml A 1-42 in the presence or absence of selective agonists and antagonists of cannabinoid (CB)1 and CB2 receptors.

    Article Title: Cannabidiol and Oxygen-Ozone Combination Induce Cytotoxicity in Human Pancreatic Ductal Adenocarcinoma Cell Lines
    Article Snippet: Lysates were separated on a SDS polyacrylamide gel, transferred onto Hybond-C extra membranes (GE Healthcare, Chicago, IL, USA), blocked with 5% low-fat dry milk in phosphate-buffered saline 0.1% Tween 20 overnight at 4 °C, immunoblotted with mouse anti-CB1 (1:500, Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-CB2 (1:200, Cayman Chemical, Ellsworth, MI, USA), mouse anti-TRPV1 (1:200, Santa Cruz Biotechnology), mouse anti-TRPV2 (1:200, Santa Cruz Biotechnology), rabbit anti-TRPV3 (0.5 μg/mL, Boster Biological Technology, Pleasanton, CA, USA), rabbit anti-TRPV4 (1:500, Assay Biotechnology Company, Fremont, CA, USA), goat anti-TRPA1 (1:300, Santa Cruz Biotechnology), rabbit anti-TRPM8 (0.5 μg/mL, Boster Biological Technology), mouse anti-glyceraldehydes-3-phosphate dehydrogenase (GAPDH, 1:3000, OriGene, Rockville, MD, USA), and rabbit anti-caspase-3 (1:1000, Cell Signaling, Danvers, MA, USA) Abs overnight or 1 h according to manufacturer’s protocol and then incubated with their respective HRP-cojugated anti-rabbit, anti-mouse (1:2000, Cell Signaling, Danvers, MA, USA) or anti-goat (1:1000, Santa Cruz Biotechnology) Abs for 1 h. Peroxidase activity was visualized with the LiteAblot ® PLUS or TURBO (EuroClone, Milan, Italy) kit and densitometric analysis was carried out by a Chemidoc using the Quantity One software version 4.6 (Bio-Rad, Milan, Italy).

    Article Title: The fatty acid amide hydrolase inhibitor oleoyl ethyl amide counteracts bladder overactivity in female rats.
    Article Snippet: Giorgio Gandaglia,* Frank Strittmatter, Giovanni La Croce, Fabio Benigni, Arianna Bettiga, Fabio Castiglione, Marco Moschini, Francesco Mistretta, Christian Gratzke, Francesco Montorsi, Christian Stief, and Petter Hedlund Urological Research Institute, San Raffaele Scientific Institute, Milan, Italy Department of Clinical and Experimental Pharmacology, Lund University, Lund, Sweden Department of Urology, Munich University, Munich, Germany Department of Clinical Pharmacology, Link€ oping University, Link€ oping, Sweden

    Article Title: The endogenous cannabinoid system in the gut of patients with inflammatory bowel disease.
    Article Snippet: Membranes were blocked with 10 % nonfat dried milk and 5 % bovine serum albumin for 2 h, and then incubated overnight with rabbit anti-CB1 (diluted 1:500, Cayman Chemicals, Ann Arbor, MI), rabbit anti-CB2 (1:300, Cayman Chemicals), and mouse anti- -actin (1:1,000), mouse anti-STAT4 (1:500), mouse anti-phospho-STAT4 (pSTAT4, 1:500), and mouse anti-T-bet (1:500), all from Santa Cruz Biotechnology (Santa Cruz, CA).

    Software:

    Article Title: Synthetic cannabinoids reduce the inflammatory activity of microglia and subsequently improve neuronal survival in vitro.
    Article Snippet: Microglia are resident immune cells of the brain that survey the microenvironment, provide trophic support to neurons, and clear debris to maintain homeostasis and healthy brain function.. Microglia are also drivers of neuroinflammation in several neurodegenerative diseases.. Microglia produce endocannabinoids and express both cannabinoid receptor subtypes suggesting that this system is a target to suppress neuroinflammation.

    Article Title: Opposing control of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis: in vitro and in vivo evidence.
    Article Snippet: Beside cytotoxic mechanisms impacting on neurons, amyloid (A )-induced astroglial activation is operative in Alzheimer’s disease brain, suggesting that persistent inflammatory response may have a role in the illness and that positive results may be achieved by curbing the astroglial reaction.. Because the role of the endocannabinoid system could represent a promising field of research, the present study conducted in vitro and in vivo experiments to assess this system.. C6 rat astroglioma cells were challenged with 1 g/ml A 1-42 in the presence or absence of selective agonists and antagonists of cannabinoid (CB)1 and CB2 receptors.

    Article Title: Cannabidiol and Oxygen-Ozone Combination Induce Cytotoxicity in Human Pancreatic Ductal Adenocarcinoma Cell Lines
    Article Snippet: Lysates were separated on a SDS polyacrylamide gel, transferred onto Hybond-C extra membranes (GE Healthcare, Chicago, IL, USA), blocked with 5% low-fat dry milk in phosphate-buffered saline 0.1% Tween 20 overnight at 4 °C, immunoblotted with mouse anti-CB1 (1:500, Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-CB2 (1:200, Cayman Chemical, Ellsworth, MI, USA), mouse anti-TRPV1 (1:200, Santa Cruz Biotechnology), mouse anti-TRPV2 (1:200, Santa Cruz Biotechnology), rabbit anti-TRPV3 (0.5 μg/mL, Boster Biological Technology, Pleasanton, CA, USA), rabbit anti-TRPV4 (1:500, Assay Biotechnology Company, Fremont, CA, USA), goat anti-TRPA1 (1:300, Santa Cruz Biotechnology), rabbit anti-TRPM8 (0.5 μg/mL, Boster Biological Technology), mouse anti-glyceraldehydes-3-phosphate dehydrogenase (GAPDH, 1:3000, OriGene, Rockville, MD, USA), and rabbit anti-caspase-3 (1:1000, Cell Signaling, Danvers, MA, USA) Abs overnight or 1 h according to manufacturer’s protocol and then incubated with their respective HRP-cojugated anti-rabbit, anti-mouse (1:2000, Cell Signaling, Danvers, MA, USA) or anti-goat (1:1000, Santa Cruz Biotechnology) Abs for 1 h. Peroxidase activity was visualized with the LiteAblot ® PLUS or TURBO (EuroClone, Milan, Italy) kit and densitometric analysis was carried out by a Chemidoc using the Quantity One software version 4.6 (Bio-Rad, Milan, Italy).

    Article Title: The fatty acid amide hydrolase inhibitor oleoyl ethyl amide counteracts bladder overactivity in female rats.
    Article Snippet: Giorgio Gandaglia,* Frank Strittmatter, Giovanni La Croce, Fabio Benigni, Arianna Bettiga, Fabio Castiglione, Marco Moschini, Francesco Mistretta, Christian Gratzke, Francesco Montorsi, Christian Stief, and Petter Hedlund Urological Research Institute, San Raffaele Scientific Institute, Milan, Italy Department of Clinical and Experimental Pharmacology, Lund University, Lund, Sweden Department of Urology, Munich University, Munich, Germany Department of Clinical Pharmacology, Link€ oping University, Link€ oping, Sweden

    Article Title: The endogenous cannabinoid system in the gut of patients with inflammatory bowel disease.
    Article Snippet: Membranes were blocked with 10 % nonfat dried milk and 5 % bovine serum albumin for 2 h, and then incubated overnight with rabbit anti-CB1 (diluted 1:500, Cayman Chemicals, Ann Arbor, MI), rabbit anti-CB2 (1:300, Cayman Chemicals), and mouse anti- -actin (1:1,000), mouse anti-STAT4 (1:500), mouse anti-phospho-STAT4 (pSTAT4, 1:500), and mouse anti-T-bet (1:500), all from Santa Cruz Biotechnology (Santa Cruz, CA).

    Nucleic Acid Electrophoresis:

    Article Title: Synthetic cannabinoids reduce the inflammatory activity of microglia and subsequently improve neuronal survival in vitro.
    Article Snippet: Microglia are resident immune cells of the brain that survey the microenvironment, provide trophic support to neurons, and clear debris to maintain homeostasis and healthy brain function.. Microglia are also drivers of neuroinflammation in several neurodegenerative diseases.. Microglia produce endocannabinoids and express both cannabinoid receptor subtypes suggesting that this system is a target to suppress neuroinflammation.

    Article Title: Opposing control of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis: in vitro and in vivo evidence.
    Article Snippet: Beside cytotoxic mechanisms impacting on neurons, amyloid (A )-induced astroglial activation is operative in Alzheimer’s disease brain, suggesting that persistent inflammatory response may have a role in the illness and that positive results may be achieved by curbing the astroglial reaction.. Because the role of the endocannabinoid system could represent a promising field of research, the present study conducted in vitro and in vivo experiments to assess this system.. C6 rat astroglioma cells were challenged with 1 g/ml A 1-42 in the presence or absence of selective agonists and antagonists of cannabinoid (CB)1 and CB2 receptors.

    Article Title: Cannabidiol and Oxygen-Ozone Combination Induce Cytotoxicity in Human Pancreatic Ductal Adenocarcinoma Cell Lines
    Article Snippet: Lysates were separated on a SDS polyacrylamide gel, transferred onto Hybond-C extra membranes (GE Healthcare, Chicago, IL, USA), blocked with 5% low-fat dry milk in phosphate-buffered saline 0.1% Tween 20 overnight at 4 °C, immunoblotted with mouse anti-CB1 (1:500, Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-CB2 (1:200, Cayman Chemical, Ellsworth, MI, USA), mouse anti-TRPV1 (1:200, Santa Cruz Biotechnology), mouse anti-TRPV2 (1:200, Santa Cruz Biotechnology), rabbit anti-TRPV3 (0.5 μg/mL, Boster Biological Technology, Pleasanton, CA, USA), rabbit anti-TRPV4 (1:500, Assay Biotechnology Company, Fremont, CA, USA), goat anti-TRPA1 (1:300, Santa Cruz Biotechnology), rabbit anti-TRPM8 (0.5 μg/mL, Boster Biological Technology), mouse anti-glyceraldehydes-3-phosphate dehydrogenase (GAPDH, 1:3000, OriGene, Rockville, MD, USA), and rabbit anti-caspase-3 (1:1000, Cell Signaling, Danvers, MA, USA) Abs overnight or 1 h according to manufacturer’s protocol and then incubated with their respective HRP-cojugated anti-rabbit, anti-mouse (1:2000, Cell Signaling, Danvers, MA, USA) or anti-goat (1:1000, Santa Cruz Biotechnology) Abs for 1 h. Peroxidase activity was visualized with the LiteAblot ® PLUS or TURBO (EuroClone, Milan, Italy) kit and densitometric analysis was carried out by a Chemidoc using the Quantity One software version 4.6 (Bio-Rad, Milan, Italy).

    Article Title: The fatty acid amide hydrolase inhibitor oleoyl ethyl amide counteracts bladder overactivity in female rats.
    Article Snippet: Giorgio Gandaglia,* Frank Strittmatter, Giovanni La Croce, Fabio Benigni, Arianna Bettiga, Fabio Castiglione, Marco Moschini, Francesco Mistretta, Christian Gratzke, Francesco Montorsi, Christian Stief, and Petter Hedlund Urological Research Institute, San Raffaele Scientific Institute, Milan, Italy Department of Clinical and Experimental Pharmacology, Lund University, Lund, Sweden Department of Urology, Munich University, Munich, Germany Department of Clinical Pharmacology, Link€ oping University, Link€ oping, Sweden

    Article Title: The endogenous cannabinoid system in the gut of patients with inflammatory bowel disease.
    Article Snippet: Membranes were blocked with 10 % nonfat dried milk and 5 % bovine serum albumin for 2 h, and then incubated overnight with rabbit anti-CB1 (diluted 1:500, Cayman Chemicals, Ann Arbor, MI), rabbit anti-CB2 (1:300, Cayman Chemicals), and mouse anti- -actin (1:1,000), mouse anti-STAT4 (1:500), mouse anti-phospho-STAT4 (pSTAT4, 1:500), and mouse anti-T-bet (1:500), all from Santa Cruz Biotechnology (Santa Cruz, CA).

    Membrane:

    Article Title: Synthetic cannabinoids reduce the inflammatory activity of microglia and subsequently improve neuronal survival in vitro.
    Article Snippet: Microglia are resident immune cells of the brain that survey the microenvironment, provide trophic support to neurons, and clear debris to maintain homeostasis and healthy brain function.. Microglia are also drivers of neuroinflammation in several neurodegenerative diseases.. Microglia produce endocannabinoids and express both cannabinoid receptor subtypes suggesting that this system is a target to suppress neuroinflammation.

    Article Title: Opposing control of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis: in vitro and in vivo evidence.
    Article Snippet: Beside cytotoxic mechanisms impacting on neurons, amyloid (A )-induced astroglial activation is operative in Alzheimer’s disease brain, suggesting that persistent inflammatory response may have a role in the illness and that positive results may be achieved by curbing the astroglial reaction.. Because the role of the endocannabinoid system could represent a promising field of research, the present study conducted in vitro and in vivo experiments to assess this system.. C6 rat astroglioma cells were challenged with 1 g/ml A 1-42 in the presence or absence of selective agonists and antagonists of cannabinoid (CB)1 and CB2 receptors.

    Article Title: Cannabidiol and Oxygen-Ozone Combination Induce Cytotoxicity in Human Pancreatic Ductal Adenocarcinoma Cell Lines
    Article Snippet: Lysates were separated on a SDS polyacrylamide gel, transferred onto Hybond-C extra membranes (GE Healthcare, Chicago, IL, USA), blocked with 5% low-fat dry milk in phosphate-buffered saline 0.1% Tween 20 overnight at 4 °C, immunoblotted with mouse anti-CB1 (1:500, Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-CB2 (1:200, Cayman Chemical, Ellsworth, MI, USA), mouse anti-TRPV1 (1:200, Santa Cruz Biotechnology), mouse anti-TRPV2 (1:200, Santa Cruz Biotechnology), rabbit anti-TRPV3 (0.5 μg/mL, Boster Biological Technology, Pleasanton, CA, USA), rabbit anti-TRPV4 (1:500, Assay Biotechnology Company, Fremont, CA, USA), goat anti-TRPA1 (1:300, Santa Cruz Biotechnology), rabbit anti-TRPM8 (0.5 μg/mL, Boster Biological Technology), mouse anti-glyceraldehydes-3-phosphate dehydrogenase (GAPDH, 1:3000, OriGene, Rockville, MD, USA), and rabbit anti-caspase-3 (1:1000, Cell Signaling, Danvers, MA, USA) Abs overnight or 1 h according to manufacturer’s protocol and then incubated with their respective HRP-cojugated anti-rabbit, anti-mouse (1:2000, Cell Signaling, Danvers, MA, USA) or anti-goat (1:1000, Santa Cruz Biotechnology) Abs for 1 h. Peroxidase activity was visualized with the LiteAblot ® PLUS or TURBO (EuroClone, Milan, Italy) kit and densitometric analysis was carried out by a Chemidoc using the Quantity One software version 4.6 (Bio-Rad, Milan, Italy).

    Article Title: The fatty acid amide hydrolase inhibitor oleoyl ethyl amide counteracts bladder overactivity in female rats.
    Article Snippet: Giorgio Gandaglia,* Frank Strittmatter, Giovanni La Croce, Fabio Benigni, Arianna Bettiga, Fabio Castiglione, Marco Moschini, Francesco Mistretta, Christian Gratzke, Francesco Montorsi, Christian Stief, and Petter Hedlund Urological Research Institute, San Raffaele Scientific Institute, Milan, Italy Department of Clinical and Experimental Pharmacology, Lund University, Lund, Sweden Department of Urology, Munich University, Munich, Germany Department of Clinical Pharmacology, Link€ oping University, Link€ oping, Sweden

    Article Title: The endogenous cannabinoid system in the gut of patients with inflammatory bowel disease.
    Article Snippet: Membranes were blocked with 10 % nonfat dried milk and 5 % bovine serum albumin for 2 h, and then incubated overnight with rabbit anti-CB1 (diluted 1:500, Cayman Chemicals, Ann Arbor, MI), rabbit anti-CB2 (1:300, Cayman Chemicals), and mouse anti- -actin (1:1,000), mouse anti-STAT4 (1:500), mouse anti-phospho-STAT4 (pSTAT4, 1:500), and mouse anti-T-bet (1:500), all from Santa Cruz Biotechnology (Santa Cruz, CA).



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    Fig. 1. Immunohistochemistry for CB1 and <t>CB2</t> in the carotid body. (A) Dot-like CB1 immunoreactivity is observed throughout the carotid body. Intense immu- noreactivity is shown in nerve fibers and/or endings (arrows). (B) A higher magnification view of the rectangle in panel A. Dot-like CB1 immunoreactivity is observed in the perinuclear cytoplasm of chemoreceptor cells (arrows). Intense immunoreactivity for CB1 is also present in nerve endings (arrowheads). (C) Dot-like CB2 immunoreactivity is noted throughout the carotid body. (D) A higher magnification view (rectangle in panel C) shows small dots of CB2 immunoreactivity in the perinuclear region of chemoreceptor cells (arrow). (E-G) Double immunofluorescence for CB1 and CB2 shows CB1-immunoreactive (arrows) and CB2- immunoreactive dots (arrow heads) in the same chemoreceptor cell; however, they are not colocalized.
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    Fig. 1. Immunohistochemistry for CB1 and <t>CB2</t> in the carotid body. (A) Dot-like CB1 immunoreactivity is observed throughout the carotid body. Intense immu- noreactivity is shown in nerve fibers and/or endings (arrows). (B) A higher magnification view of the rectangle in panel A. Dot-like CB1 immunoreactivity is observed in the perinuclear cytoplasm of chemoreceptor cells (arrows). Intense immunoreactivity for CB1 is also present in nerve endings (arrowheads). (C) Dot-like CB2 immunoreactivity is noted throughout the carotid body. (D) A higher magnification view (rectangle in panel C) shows small dots of CB2 immunoreactivity in the perinuclear region of chemoreceptor cells (arrow). (E-G) Double immunofluorescence for CB1 and CB2 shows CB1-immunoreactive (arrows) and CB2- immunoreactive dots (arrow heads) in the same chemoreceptor cell; however, they are not colocalized.
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    Image Search Results


    Fig. 1. Immunohistochemistry for CB1 and CB2 in the carotid body. (A) Dot-like CB1 immunoreactivity is observed throughout the carotid body. Intense immu- noreactivity is shown in nerve fibers and/or endings (arrows). (B) A higher magnification view of the rectangle in panel A. Dot-like CB1 immunoreactivity is observed in the perinuclear cytoplasm of chemoreceptor cells (arrows). Intense immunoreactivity for CB1 is also present in nerve endings (arrowheads). (C) Dot-like CB2 immunoreactivity is noted throughout the carotid body. (D) A higher magnification view (rectangle in panel C) shows small dots of CB2 immunoreactivity in the perinuclear region of chemoreceptor cells (arrow). (E-G) Double immunofluorescence for CB1 and CB2 shows CB1-immunoreactive (arrows) and CB2- immunoreactive dots (arrow heads) in the same chemoreceptor cell; however, they are not colocalized.

    Journal: Acta histochemica

    Article Title: Immunohistochemical distribution of cannabinoid receptor type 1 (CB1) and type 2 (CB2) in the rat carotid body.

    doi: 10.1016/j.acthis.2024.152205

    Figure Lengend Snippet: Fig. 1. Immunohistochemistry for CB1 and CB2 in the carotid body. (A) Dot-like CB1 immunoreactivity is observed throughout the carotid body. Intense immu- noreactivity is shown in nerve fibers and/or endings (arrows). (B) A higher magnification view of the rectangle in panel A. Dot-like CB1 immunoreactivity is observed in the perinuclear cytoplasm of chemoreceptor cells (arrows). Intense immunoreactivity for CB1 is also present in nerve endings (arrowheads). (C) Dot-like CB2 immunoreactivity is noted throughout the carotid body. (D) A higher magnification view (rectangle in panel C) shows small dots of CB2 immunoreactivity in the perinuclear region of chemoreceptor cells (arrow). (E-G) Double immunofluorescence for CB1 and CB2 shows CB1-immunoreactive (arrows) and CB2- immunoreactive dots (arrow heads) in the same chemoreceptor cell; however, they are not colocalized.

    Article Snippet: CB2: A rabbit polyclonal anti-CB2 antibody (NB300–606, Novus Biologicals, Centennial, CO, U.S.A.) was raised from a fusion protein that contained the first 33 amino acid residues of human CB2.

    Techniques: Immunohistochemistry, Immunofluorescence

    Fig. 2. (A-C)Triple immunofluorescence for CB1 with TH and DBH. CB1-immunoreactive dot-like structures are observed in both TH- (arrows) and DBH- immunoreactive chemoreceptor cells (arrowheads). Dot-like immunoreactivity for CB1 localizes in both the perinuclear cytoplasm and outlines of the regions of immunoreactivity for TH or DBH. (D-F) Triple immunofluorescence for CB2 with TH and DBH. CB2 immunoreactivity is shown in the perinuclear cytoplasm of TH- (arrows) and DBH-immunoreactive chemoreceptor cells (arrowheads).

    Journal: Acta histochemica

    Article Title: Immunohistochemical distribution of cannabinoid receptor type 1 (CB1) and type 2 (CB2) in the rat carotid body.

    doi: 10.1016/j.acthis.2024.152205

    Figure Lengend Snippet: Fig. 2. (A-C)Triple immunofluorescence for CB1 with TH and DBH. CB1-immunoreactive dot-like structures are observed in both TH- (arrows) and DBH- immunoreactive chemoreceptor cells (arrowheads). Dot-like immunoreactivity for CB1 localizes in both the perinuclear cytoplasm and outlines of the regions of immunoreactivity for TH or DBH. (D-F) Triple immunofluorescence for CB2 with TH and DBH. CB2 immunoreactivity is shown in the perinuclear cytoplasm of TH- (arrows) and DBH-immunoreactive chemoreceptor cells (arrowheads).

    Article Snippet: CB2: A rabbit polyclonal anti-CB2 antibody (NB300–606, Novus Biologicals, Centennial, CO, U.S.A.) was raised from a fusion protein that contained the first 33 amino acid residues of human CB2.

    Techniques: Immunofluorescence

    Fig. 3. (A-C) Double immunofluorescence for CB1 with P2X3. Intense CB1 immunoreactivity is surrounded by P2X3 immunoreactivity in the sensory nerve endings around chemoreceptor cells (arrows). (D-F) Double immunofluorescence for CB1 with VGluT2. CB1 immunoreactivity colocalizes with VGluT2 immunoreactivity, and appears to be in close contact with chemoreceptor cells (arrows). (G-H) Double immunofluorescence for CB2 with P2X2. Weak CB2-immunoreactive dots localize within P2X2-immunoreactive sensory nerve endings (arrows).

    Journal: Acta histochemica

    Article Title: Immunohistochemical distribution of cannabinoid receptor type 1 (CB1) and type 2 (CB2) in the rat carotid body.

    doi: 10.1016/j.acthis.2024.152205

    Figure Lengend Snippet: Fig. 3. (A-C) Double immunofluorescence for CB1 with P2X3. Intense CB1 immunoreactivity is surrounded by P2X3 immunoreactivity in the sensory nerve endings around chemoreceptor cells (arrows). (D-F) Double immunofluorescence for CB1 with VGluT2. CB1 immunoreactivity colocalizes with VGluT2 immunoreactivity, and appears to be in close contact with chemoreceptor cells (arrows). (G-H) Double immunofluorescence for CB2 with P2X2. Weak CB2-immunoreactive dots localize within P2X2-immunoreactive sensory nerve endings (arrows).

    Article Snippet: CB2: A rabbit polyclonal anti-CB2 antibody (NB300–606, Novus Biologicals, Centennial, CO, U.S.A.) was raised from a fusion protein that contained the first 33 amino acid residues of human CB2.

    Techniques: Immunofluorescence

    qPCR evaluation of the cannabinoid receptor expression in the model cell lines. HL60 and LN229 cell lines were used as a reference with a high expression of CB2 and CB1 and GPR55 receptor pair, accordingly.

    Journal: International Journal of Molecular Sciences

    Article Title: The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells

    doi: 10.3390/ijms25042271

    Figure Lengend Snippet: qPCR evaluation of the cannabinoid receptor expression in the model cell lines. HL60 and LN229 cell lines were used as a reference with a high expression of CB2 and CB1 and GPR55 receptor pair, accordingly.

    Article Snippet: The following antibodies were used: rabbit anti-GPR55 (Abcam ab203663), rabbit anti-CB2 (Abcam ab45942), rabbit anti-CB1 (Abcam ab23703), and mouse anti-beta-actin (Abcam ab8226); and secondary antibodies (coupled to alkaline phosphatase) anti-rabbit IgG (Sigma-Aldrich A9919) and anti-mouse IgG (Jackson ImmunoResearch 111-055-003).

    Techniques: Expressing

    The effect of receptor blockers on the effect of AEA’s combination with LPI on breast cancer cell lines’ viability. The following substances and concentrations were used: CB1, SR 141716A (100 nM); CB2, SR 144528 (100 nM); GPR55, ML-193 (2 µM); GPR18, PSB CB5 (3 µM). Incubation time: 72 h; resazurin test, mean ± standard error ( n = 4 experiments). ( A ) MCF-10A, ( B ) MCF-7, ( C ) BT-474, ( D ) SK-BR-3, ( E ) BT-20, ( F ) MDA-MB-231.

    Journal: International Journal of Molecular Sciences

    Article Title: The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells

    doi: 10.3390/ijms25042271

    Figure Lengend Snippet: The effect of receptor blockers on the effect of AEA’s combination with LPI on breast cancer cell lines’ viability. The following substances and concentrations were used: CB1, SR 141716A (100 nM); CB2, SR 144528 (100 nM); GPR55, ML-193 (2 µM); GPR18, PSB CB5 (3 µM). Incubation time: 72 h; resazurin test, mean ± standard error ( n = 4 experiments). ( A ) MCF-10A, ( B ) MCF-7, ( C ) BT-474, ( D ) SK-BR-3, ( E ) BT-20, ( F ) MDA-MB-231.

    Article Snippet: The following antibodies were used: rabbit anti-GPR55 (Abcam ab203663), rabbit anti-CB2 (Abcam ab45942), rabbit anti-CB1 (Abcam ab23703), and mouse anti-beta-actin (Abcam ab8226); and secondary antibodies (coupled to alkaline phosphatase) anti-rabbit IgG (Sigma-Aldrich A9919) and anti-mouse IgG (Jackson ImmunoResearch 111-055-003).

    Techniques: Incubation